UNIT SEVEN:  MITOSIS AND MEIOSIS; ASEXUAL AND SEXUAL REPRODUCTION

Upon completion of this unit you will be able to:

1.  Explain the cell cycle and identify the significant features of the G1, S and G2 phases
2.  Name the stages of mitosis and describe major events that distinguish each stage.
3.  List cells that lose their mitotic ability and those that retain this capacity.
4.  Cite prominent differences between plant and animal cells during mitosis.
5.  Describe the characteristics of a clone and explain how it is produced.
6.  Compare and contrast mitosis and meiosis.
7.  Explain the importance of crossing-over during meiosis.
8.  Explain why sexually reproducing organisms must form cells with a haploid number of  chromosomes.
9.  Distinguish between sexual and asexual reproduction.
10.  Cite examples of asexual reproduction displayed by living organisms.
11.  List advantages and disadvantages of sexual and asexual reproduction.
12.  Define Nondisjunction and explain its relavance to chromosomal genetics. 
13.  Identify the four flower parts, provide their functions and describe which are essential flower parts.
14.  Differentiate between pollination and fertilization.
15.  List several mechanisms of pollination.
16.  Discuss alternation of generation and distinguish between sporophyte and gametophyte  generations.
17.  Define dioecious, monoecious and synoecious and site examples of plants following each strategy.
18.  Identify the plumule, radicle, and cotyledon in the seed embryo.
19.  Discuss the development of a fruit;  describe the different classifications of fruit.
20.  Differentiate between parthenogenesis and parthenocarpy.
21.  Describe hermaphroditic animals.
22.  List examples of oviparous, viviparous and ovoviviparous animals.
23.  Discuss the efficiency issues facing animals using external and internal fertilization strategies.
24.  Compare and contrast the embryo of a seed plant with that of an oviparous animal.
25.  Provide the functions of the following:  testes, testosterone, estrogen, follicle, corpus luteum,  progesterone, pituitary gland uterus, FSH, LH, ovulation.
 

Unit References:  Text Chapters 9, 22, 27  http://www.mhhe.com/enger12

                                An Online Biology Text Book

Unit 7:  Mitosis/Meiosis; Sexual/Asexual Reproduction
(how genetic information is passed)

Biological Trivia:  (Objective #3)

     -Average RBC lives ~120 days and there are about 25 trillion in an adult.  To maintain,
        about 2.5  million new RBC's must be produced by each second

     -Nerve cells lose their capacity to replicate once they mature

     -Some cells retain capacity to divide but do so only under certain circumstances:

    Liver- if portion removed surgically remaining cells will replicate themselves until liver
        reaches  its former size.
     -About 2 trillion cell divisions occur in an adult human every 24 hours, that's ~25 million/sec.

I.  MITOSIS/MEIOSIS  (Objective #6)   pg 164-171 of text
     A. Mitosis - nuclear division, maintains chromosome number unchanged
            from  parent
         -occurs in somatic cells
          -occurs in bacteria (binary fission) and single celled animals
          -daughter cells have same genetic makeup as parent cells
          -growth/ repair and maintenance

            Check Out :   The Cell Cycle and Mitosis Tutorial

                                    Onion Root Tip Simulation
                                    Interactive Mitosis Tutorial
                                   
                                    Shockwave Mitosis

     B. Meiosis pg.  174-181- nuclear division, chromosome # reduced from diploid to
            haploid   (Objective #8)
          -sexually reproducing organisms, produces variability in chromosome sets
          -crossing over produces  greater variability
          -homologous chromosomes pair.

     C. Cell Cycle (Objective #1, 2) text pg 164-170
          Interphase:
                G1- Gap phase one; cell grows, produces tRNA, mRNA, ribosomes, enzymes
                S-  synthesis; DNA replication, prep for cell division
                G2- Gap phase two; synthesis of fibers needed to pull chromosomes apart
                         

          Text pgs. 166-169
          Mitosis:   Prophase; chromatin becomes condensed to chromosomes
                          Metaphase; chromosomes line up at equator
                          Anaphase; chromosomes split at centromere and move to poles
                          Telophase; new sets of chromosomes becomes enclosed by new nuclear
                                               membrane

                        Cytokinesis- division of cytoplasm

          *gradual and continuous process

          *nerve and muscle cells come to rest in S phase

(Objective #4) text pg 169
          *Plant cells- cell plate forms between new cells
          *Animal cells - have centrioles, cleavage furrow forms between cells

          Meiosis- (pgs 174-181 )  2 divisions rather than one as in mitosis
                (prophase I . .  prophase II. .
               In mitosis all chromosome pairs line up on equator

               In meiosis homologous chromosomes line up (2 lines)

          Mitosis                           Meiosis
 
 
              Check Out :   Botany Online- Meiosis (Check Biology Handbook)
                                     NCSU Meiosis Tutorial
                                     How Cells Divide: Mitosis vs. Meiosis
                                     Biology Hypertextbook: Meiosis and Genetic Recombination

                                     Biology Project Meiosis Tutorial
  
     CROSSING OVER (Objective #7)  text pg 182-183   events in Meiosis increase genetic variability  (allows page by page variability)

          w/o crossover
 

          w/ crossover 
               
            Check Out :   Crossing Over of Chromosomes in Meiosis


Problems with Meiosis: Nondisjunction  (objective 12) , text pg 185-187

    Downs Syndrome- autosomal nondisjunction
    Kleinfelters, Turners- sex chromosome nondisjunction

        Nondisjunction in autosomes rarely tolerated

II  SEXUAL AND ASEXUAL REPRODUCTION   text pgs 164 (Objective #9, 10)
       A. Asexual reproduction - **main reproductive event is mitosis, one parent (single
            donor of  genetic information), end result is two genetically identical offspring
            cells (daughter cells)

          1.  Fission- bacteria, single celled animals, yeast, algae
               -nuclear material replicates and then organism divides
               -2 identical daughter cells produced

          2.  Budding- small projection of cellular material contains DNA, grows from cell
                (or organism):   yeast, hydra
               -as bud grows wall develops between bud and parent, seperation occurs, new
                individual arrives

          3.  Fragmentation/regeneration
               -new organisms formed from pieces; anemonies, sponges
               -starfish can split central disc; Crown of Thorns (at least 1/5 central disc)

          4.  Sporulation- fungi, (some plants), protozoans, bacteria
               spore- reproductive cell (mitosis), protective coat, widely dispersed.  Contains
                            nuclear  material
               -mildew, plant rusts, smuts, difficult to destroy

          5.  Vegetative- plants (best known)
               -production of new organisms or portion from part of a living organism.
               -plant cuttings, potatoes, strawberries (runners, stolons), grafts (trees)

          ADVANTAGES OF ASEXUAL REPRODUCTION  (Objective  11)
               -simple process, one parent involved, no waste of energy to find mate
               -large numbers can be produced
               -spores can be widely dispersed, increases chance of survival

          DISADVANTAGES OF ASEXUAL REPRODUCTION
               -produces clones.  In an environment that changes there is no variability to deal
                w/changes
   
             Check Out:   Asexual Reproducers

                                     Breakthroughs in Bioscience- Cloning

        B. Sexual Reproduction pg 174 (Objective #9) - requires haploid gametes (eggs and
            sperm) that unite  to produce a  zygote
          -main reproductive event is meiosis (gamete production)

          1. ADVANTAGES (Objective #11)
               -introduction of variability to zygote from two parents.  Allows greater diversity and
                adaptability
               -care of young

          2. DISADVANTAGES
               -2 kinds of gametes required
               -gametes must be brought together, usually deals with large #s of gametes under
                relatively   "iffy" conditions
                    Ex. Human egg production vs. Fish egg production;  Human infertility  

            Check Out :   Why Sex? 
 

III.  Plant Reproduction  (Higher plants- flowering plants)  (Objective #13) text pg 175, 474-484
          1.  Flower Parts pg 507
               -Pistil (female part of flower) *essential flower part
                    Stigma (pollen receptor)

                    Style (tube from stigma to ovary)

                    Ovary (haploid spores or eggs)

               -Stamen (male part of flower)  *essential flower part
                    Anther (haploid pollen spores)

                    Filament (stalk on which anther resides)

                   Mature pollen contain 2 nuclei:
                              -generative nucleus ---> sperm

                              -tube nucleus ---> directs growth of pollen tube

               -Petals- (*non essential flower part)  protect inner reproductive organs and
                    attract insects
               -Sepals- (*non essential flower part)  protects flowers when they are in bud stage

(Objective #14, 15)
  **Pollination- transfer of pollen from male to female
          Various strategies:  ~65% of all flowering plants are insect pollinated

          -grasses, wind; ferns, mosses, water; animal carriers

     **Fertilization- union of egg and sperm
 

(Objective #17)
When dealing with stamens and pistils (and how best to get pollen together with ovum)
there are three strategies:
     Monoecious plants- both male and female flower on same plant
               Corn: male tassels (stamens only), female silks (pistil only)

     Dioecious plants- separate sexes, therefore, separate plants
               Cottonwood, honey locus, (humans)

     Synoecious plants- male and female parts in same flower
               Most "showy" flowers

ALTERNATION OF GENERATIONS  (Objective #16)  text pg 475
          *microspore (male, produced by mitosis) grow into male gametophyte or stage of
            life cycle  that produces pollen/sperm (meiosis)

          *megaspore (female, produced by mitosis) grow into female gametophyte or stage
            of life cycle  that produces egg/ovum (meiosis)

Alternation between: **Asexual and Sexual stages
**A haploid and a diploid
  A spore producer (sporophyte)and a gamete producer       (gametophyte)

     1.  Mature male gametophyte ---> produces pollen grain (1N) (releases sperm)
          Female gametophyte retained in flower and produces ovum (1N)

     2.  Fertilization results in an embryo (2N zygote) that is eventually enclosed as a
            seed and  covered by a fruit

          -fertilization double  --> generative nucleus (pollen) + egg (ovum) = embryo (2N)

                                  --> tube nucleus + polar cells = endosperm

    Endosperm- nutrient material for seed; Polar cells (2 of seven nuclei in embryo sac,
    other 5  absorbed)
 

Seed  (Objective #18) text pg 482-483- structure formed by the maturation of the ovule after fertilization

     Contains:  1. sporophyte embryo
                       2. stored food (endosperm)

     **Fruit is a mature ovary that surrounds and protects seed

                                                                        Seed Coat- protective

                                                                         Cotyledon- food storage
 

                                                                      Radicle- root origin
 
 
 

                                                     Plumule- shoot
          Bean Seed (dicot)

Fruits  (Objective #19) text pg 482- ovary wall thickens and becomes pericarp
     *Simple fruits- single pistil w/ one to many ovum
          Fleshy pericarp
            Simple ovary, one seed (drupe)- peach, plum, cherry
            Compound ovary, w/many seeds (berry)- orange, tomato, grape
            Compound ovary, w/many seeds seperated into wedges (hisperidium)-  orange
            Compound ovary, flesh from recepticle (pome)- apple, pear

          Dry pericarp
               usually simple ovary that splits in various ways; milkweed, beans, peas,
                sunflower, grains
         *Compound fruits- develop from groups of individual ovaries
          -aggregate fruits- ovaries from single flower (blackberries, strawberries, raspberries)
          -multiple fruit- ovaries from separate flowers clustered together (pineapple)

B.  Animal Reproduction  pg 175, 596-608 (chapter 27)
      Male- spermatogenesis (testes, sperm) testosterone
      Female- oogenesis (ovary, egg) estrogen

     Various Strategies- some involve great expenditures of energy just to find a mate!
        1.  Hermaphroditism  (Objective #21) - organism contains both male and female parts
               self-fertilization (rare)- Hydra
               Cross-fertilization- must find mate despite having both sexes (earthworms)

        2.  Parthenogenesis  (Objective #20)- unfertilized eggs develop into adults
               Bees- haploid males (chromosome # determines sex)
               Aphids-
               Parthenocarpy (plants)- fruit development without fertilization

(Objectives #22, 23)
        3.  External Fertilization- eggs deposited, sperm shed over eggs. Least efficient
               Numbers game.  Little control over fate of eggs

               Oviparous- "egg producing"- eggs released, fertilization and development external

        4.  Internal Fertilization- sperm deposited inside receptor of female

               Ovoviviparous- "live egg producing"
                     eggs fertilized and develop within female, nourishment in egg, hatch within
                        parent or immediately after laying.   (pit vipers, guppies, birds)

               Viviparous- "alive producing"- eggs develop within female body, nutritional aid from
                    mother (placentals).

(Objective #25) text pg 599-608
Hormonal Control of Human Reproduction
Female:  Anterior lobe of pituitary gland --->    FSH (Follicle Stimulating Hormone).
            One follicle per menstral cycle.  Follicle contains egg.

        Follicle produces estrogen

        effects hypothalamus and pituitary so that FSH production
        slows and LH (Leutinizing Hormone) released by pituitary

        LH causes follicle to mature and rupture (ovulation)
        Corpus Luteum (ruptured follicle) makes progesterone, helps prepare uterus
            for pregnancy

               If fertilization                 No fertilization
               9 months ---> baby             progesterone drops, menstral flow, repeat cycle
 

Feedback Inhibitors
     "pill"-  estrogen, inhibits FSH release, therefore, follicle never stimulated to form

              progesterone, reduces amount of LH, therefore, prevents ovulation

OBJECTIVE #24
                                  Oviparous embryo               Seed embryo
     production           large #s                                 large #s depending on species

     protection            little if any                              seed coat

     development      begins upon fertilization       begins at fertilization but experiences a
                                 continues through birth         dormant period prior to germination

     dispersal        little, usually stay in                   large, winds and animals contribute to
                             one spot to develop                 wide dispersal of seeds away from parent.
 

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